In Vivo Genotoxicity and Toxicity Assessment of Sterigmatocystin Individually and in Mixture with Aflatoxin B1

Mycotoxins are natural food and feed contaminants produced by several molds. The primary mode of exposure in humans animals is through mixtures. Aflatoxin B1 (AFB1) sterigmatocystin (STER) structurally related mycotoxins that share the same biosynthetic route. Few vivo genotoxicity assays have been performed with STER. In present study, Wistar rats were dosed orally STER (20 mg/kg b.w.), AFB1 (0.25 b.w.) or a mixture both an integrated micronucleus (bone marrow) comet study (liver kidney). was at highest feasible dose corn oil. No increase percentage micronuclei bone marrow observed any condition. Slight DNA damage detected livers treated (DNA strand breaks Fpg (Formamidopyrimidine glycosylase)-sensitive sites, respectively). Plasma, liver, kidney samples analyzed LC-MS/MS demonstrating to mycotoxins. General toxicity parameters (organs absolute weight, biochemistry, histopathology) not altered either individually mixture. overall absence individual did allow us set type interaction However, possible toxicokinetic observed.